Genomix4Life provides Next-Generation Sequencing (NGS)
services using different platforms to Universities, Research Organizations,
Public and Private Authorities.
Key applications include:
Whole genome sequencing
The complete genome sequencing makes it possible to characterize the whole DNA sequence (DNA-Seq) of an organism of any size and complexity. The assembly of a de novo genome or a re-sequenced genome can be fundamental in the discovery of SNPs, insertions, deletions and large-scale rearrangements.
Targeted re-sequencing
The re-sequencing of target regions is an effective tool
to quickly interrogate particular genomic regions of interest,
from single genes to the whole exome, across a large number
of samples. Regions of interest can be targeted by either
amplifying the target genomic regions with primers (amplicon sequencing)
or capturing these regions with probes (targeted enrichment).
This approach enables systematic detection of germline and somatic variants,
single nucleotide polymorphisms (SNPs), structural variants
and copy number variations.
Transcriptome analyses (RNA sequencing - small RNA sequencing)
The study of the transcriptome is crucial to understand the functional elements of the genome, to identify different types of transcripts (mRNAs, small and long non-coding RNAs, etc.) and to determine their expression level and their structure (terminations to the 5' and 3', exon-intron structure, alternative splicing, etc.). RNA sequencing is also suitable for comparative analysis of expressed transcripts between multiple samples.
Metagenomics
To study microbial communities directly in their natural environment
through whole genome sequencing or targeted approaches.
Epigenomics and Gene regulation (ChIP-Seq - Methyl-Seq - BS-Seq)
For studies focusing on transcriptional gene regulation, aiming to identify,
for example, protein binding sites or hypo- and hyper-methylated regions
within the genome. The methylation assays, for example, provide
a quantitative measurement of methylation at single CpG level,
offering the highest resolution for understanding epigenetic changes.
Single cell sequencing
Recent technical advances enable single-cell genome and transcriptome
sequencing methods, that are generating a fresh wave of biological
insights into development, cancer, neuroscience and many other fields,
with increasing applications in all areas of functional and medical genomics
The choice of the platform to be used depends on multiple factors,
such as specific applications, number of samples, species, etc.
The more suitable technology is to be selected based on the
specific needs of the research project. We can offer from
a full service (from library preparation to sequencing)
to sequencing of libraries prepared by customers.
